University Of Agricultural Sciences, Bangalore

Contact Details
Dr. M. Byre Gowda
Pigeonpea & Dolichos Breeder
University of Agricultural Sciences
GKVK, Bangalore - 560 065
+91 80 22736043
+91 9741414657

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Supporting research & education in the biological sciences.

The Kirkhouse Trust was set up in September 2000. It is a small Scottish charity (No. SC 030508), funded by gift aid donations from Oxford Gene Technology IP Ltd, a UK company founded by Professor Sir Ed Southern. more...

Dolichos Bean - Lablab purpureus (L.) Sweet

Crop Improvement > Tissue Culture

Tissue Culture

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Tony Philip (1982): Induced tetraploidy in Dolichos Lablab L.
Seeds of Dolichos Lablab L were germinated in petridishes and the seedlings were treated with 0.3% colchicine for about 12 hours. They were later transplanted to pots. Root tips collected from controlled and treated plants were pretreated with 0.2% colchicine for one hour and fixed in 1:3 acetic alcohol and stained in 2% aceto-orcein and 1 N Hcl mixture (9:1). Several metaphase plates from different roots tips of treated plants were examined and found to contain 48 chromosomes, varying in length from 0.67 m to 1.5 m.
Stomatal features of the treated plants were studied and compared with those of the diploids. The tetraploid plant was characterized by its large epidermal cells and stomata as compared with that of diploids. However, the stomatal index and average number of stomata per unit area were much less in the tetraploid plant.
Raju, et al., (1983): Antibiotic -promoted rooting in excised hypocotyl cuttings of Lablab.
Seeds of Lablab cv. CO. 9 were sown and seedlings were raised in the Botanical Garden of TNAU, coimbatore. The 12-day old seedlings were cut at the region of the hypocotyl at a uniform length of 10 cm. The cuttings were divided into 22 batches of 10 each. One batch was kept as control by dipping the basal parts of the cuttings in distilled water.Explants of the other batches were subjected to treatment for 48 hours with different concentrations of antibiotics and IAA. After treatment, all the explants were transferred to test tubes (10.0 x 1.5 cm)containing distilled water. They were maintained in the laboratory for another 14 days, changing water on alternate days. The test tubes were wrapped with black paper with projecting shoot portions exposed to diffused light in the laboratory. The average temperature during the experimental period ranged from 21.7 to 28.6C.
All the antibiotics employed stimulated the formation of adventitious roots on the excised cuttings compared to control. Low concentration (0.1 mg / ml) of roscullin and high concentration of (10 mg/ ml) of chloramphenicol were found to promote more number of roots compared to IAA treatments. Excepting chloramphenicol, the lower concentration (0.1 and 1mg/ml) of the other employed antibiotics promoted the development of more adventitious roots than their high concentration (10 mg / ml).
Kaushik and Khanna, (1990): Flownoides from in vivo and in vitro tissue cultures of Dolichos LablabL. and their antifungal screening.
The quercetin and kaempherol contents of the seeds, stems, leaves, flowers and morganized callus tissue of D. Lablab were determined. Both flavonoides were active against Candida albicans and Penicillium puberulum in paper disc tests.
Guleria (2013): Development of an efficient in vitro regeneration protocol of Dolichos (Lablab purpureus L.)..
This paper presents an efficient in vitro regeneration protocol for Lablab purpureus cv. HA-4. Shoot tip and cotyledonary nodal explants were cultured on MS basal media containing different combinations of growth hormones: BAP [benzyladenine], thidiazuron and NAA. The shoot tip explant resulted in higher number of healthy shoots when cultured on medium supplemented with BAP along with activated charcoal and augmentin, which played a role in rooting.
Kabir and Sen, (1990): In vitro induction and maintenance of callus in Lablabbean.
In vitro callus induction was studied using epicotyls and cotyledons from Lablab niger (L. purpureus) cv. JDL 53 grown on 3 different media supplemented with NAA, 2, 4D and Kinetin. Murashige and Skoogs (MS) medium was the sutiable. Cotyledons showed the best response with 4 mg 2, 4D mg 2, 4D + 1 mg Kinetin/ litre. Callus growth was maintained with 130.2 mg Na2Fe EDTA/litre supplemented with 2, 4D and Kinetin. Development of browning could be avoided by using 200 ml coconut milk/ litre instead of Kinetin and 200 mg casein hydrolysate/ litre as an adjuvant to MS medium.
Raj, et al., (1991): In vitro studies in Dolichos lablab.
MS medium supplemented with 2 mg 2, 4 D/ litre and 10% coconut milk produced maximum callusing from all explants. Shoot growth was obtained from shoot tips and axillary buds on most MS + growth regulator combinations. The highest percentage of regenerated plants in vitro was from nodular explants with axillary buds on MS medium supplemented with 2mg IAA/ litre or 0.5 mg IAA + 0.10.3 mg Kinetin/ litre.
Nangia and Varghese (1994): Some metabolic changes in the differentiating androgenic callus of Lablab purpureus (Linn) Sweet.
Lablabpurpureus cv. HD 105 anthers containing microspores at the uninucleate stage produced callus on PC L2 basal medium supplemented with 0.5 mg 2, 4 D, 0.5 mg NAA and 1.0 mg NAA and 2.0 mg BA/litre. Prior to rhizogenesis, the differentiating callus had higher levels of proteins and starch than after root formation. During root formation, there were higher levels of soluble sugars and the amino acids as well as higher levels of proteinase, amylase, invertase (beta fructofuranosidae) and peroxidase activity.
Muthu and Narayanasamypillai (2003): A Protocol for Efficient Plantlet Regeneration from Leaf Derived Callus of Lablab Bean (Lablab purpureus var. lignosus (L) prain)..
An efficient shoot bud differentiation and multiple shoot induction from leaf derived callus of Lablab bean (Lablab purpureus var. lignosus) have been obtained. Callus induction and shoot multiplication at various frequencies were observed using different concentrations and combinations ofauxins (IAA, 2, 4-D and NAA) and cytokinin (RAP). The highest frequency of callus induction was observed on MS medium containing 2, 4-D (3.0 mg/1) and RAP (0.5 mg/1). The green compact nodular calli occurred on NAA (3.0 mg/1) and BAP (0.5 mg/1). Highest percentage of shoot bud formation and multiplication was obtained from a combination of RAP (2.0 mg/1) and NAA (0.5 mg/1). The regenerated shoots were transferred to MS medium containing IBA (1.5 mg/1) for the induction of roots. Rooted plants were transferred to plastic cups and subsequently these were successfully transferred to fields.
Awasthi and Nautiyal, (2005): Growth response of fertilized hyacinth bean (Dolichos LablabL.) ovules to different culture media.
Fertilized ovules of hyacinth bean (Dolichos Lablab[Lablab purpureus]) were cultured after 6, 8, 10 and 12 days of anthesis in 10 different culture media devised earlier for the culture of pollinated ovaries or ovules or developing embryos in MS medium with modifications. Growth response, measured after 10 days in culture, as increase in length, breadth, fresh and dry weight, concentration of sugars, starch and protein was maximum in Monnier's medium in ovules cultured at 8 days after anthesis. Ovules cultured at 6 days after anthesis aborted within 3-4 days of culture.
Chen Yi-ming and Wang Fu-xiong (2013): The Ultrastructure of Mature Embryo Sac in Dolichos lablab.
The ultrastructure of the egg and the central cell in Dolichos lablab was investigated. When the embryo sac is mature, cell wall is formed only at the micropylar end of the egg and the synergids. At the chalazal end, no cell wall exists between the egg and the synergid, between the egg and the central coll, between the synergid and the central cell, and the plasma membranes of the adjacent cells are very close to each other. In some regions between the plasma membrane of the egg and that of the central cell, some moderate electron dense substances are present. Many mitochondria and plastids are present in the egg cytoplasm, but ER and dictyosomes are few. At the micropylar end of the synergid an elaborate filiform apparatus is distinct, and many ER tubules are dispersed in the cytoplasm of the micropylar end. It shows that the synergid would have a secretory function. The synergid contains numerous rough ER in the cytoplasm at the chalazal end, and between the plasma membrane of the egg and that of the synergid there are some vesiculae, indicating active metabolite transport between two cells. The central cell displays a high metabolic activity since numerous mitochondria, dictyosomes and ER are present in its cytoplasm. Its cell wall ingrowths form a lot of finger-like projections penetrating into the cytoplasm, which displays the feature of the transfer cell. There are many lipid bodies in the peripheral, cytoplasm. It is suggested that the central cell would play an important role in the nutrition of the embryo sac.